"scratch it out": Carbon copy based paper devices for microbial assays and liver disease diagnosis

Amrutha Hasandka, Anusha Prabhu, Akshata Prabhu, Hardik Ramesh Singhal, Giri Nandagopal M. S., Revathi Shenoy, Naresh Kumar Mani*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

We present a facile paper-based microfluidic device fabrication technique leveraging off-the-shelf carbon paper for the deposition of hydrophobic barriers using a novel "stencil scratching"method. This exceedingly frugal approach (0.05$) requires practically no technical training to employ. Hydrophobic barriers fabricated using this approach offer a width of 3 mm and a hydrophilic channel width of 849 μm, with an ability to confine major aqueous solvents without leakage. The utility of the device is demonstrated by porting a cell viability assay showing a limit-of-detection (LOD) of 0.6 × 108 CFU mL-1 and bilirubin assay with human serum showing a detection range of 1.76-6.9 mg dL-1 and a limit-of-detection (LOD) of 1.76 mg dL-1. The intuitiveness and economic viability of the fabrication method afford it great potential in the field of point-of-care diagnostics geared towards providing testing infrastructure in resource-scarce regions globally.

Original languageEnglish
Pages (from-to)3172-3180
Number of pages9
JournalAnalytical Methods
Volume13
Issue number28
DOIs
Publication statusPublished - 28-07-2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • General Chemical Engineering
  • General Engineering

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