Abstract
We present a facile paper-based microfluidic device fabrication technique leveraging off-the-shelf carbon paper for the deposition of hydrophobic barriers using a novel "stencil scratching"method. This exceedingly frugal approach (0.05$) requires practically no technical training to employ. Hydrophobic barriers fabricated using this approach offer a width of 3 mm and a hydrophilic channel width of 849 μm, with an ability to confine major aqueous solvents without leakage. The utility of the device is demonstrated by porting a cell viability assay showing a limit-of-detection (LOD) of 0.6 × 108 CFU mL-1 and bilirubin assay with human serum showing a detection range of 1.76-6.9 mg dL-1 and a limit-of-detection (LOD) of 1.76 mg dL-1. The intuitiveness and economic viability of the fabrication method afford it great potential in the field of point-of-care diagnostics geared towards providing testing infrastructure in resource-scarce regions globally.
Original language | English |
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Pages (from-to) | 3172-3180 |
Number of pages | 9 |
Journal | Analytical Methods |
Volume | 13 |
Issue number | 28 |
DOIs | |
Publication status | Published - 28-07-2021 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Chemical Engineering(all)
- Engineering(all)