TY - JOUR
T1 - Screening of Natural Stains from Indian Plants in Rat Skin Tissue for Histological Applications
AU - Jasphin, Shiny
AU - Raghavendra, Archana Parampalli
AU - Solomon, Monica Charlotte
AU - Amuthan, Arul
AU - Singh, Brij Mohan Kumar
AU - Kairanna, Nikitha Valerina
AU - Singh, Varun Kumar
AU - Sudharani, T.
N1 - Funding Information:
Acknowledgement-This work was supported by the Health India Foundation, Chennai. Grant number UTN MAHE/RG1319001”.
Publisher Copyright:
© 2022 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Histopathological diagnosis involves examination of slides stained by chemical stains which are health hazard to researchers, technicians and pathologists. This study screened plant based natural stains for histological applications. About 176 test staining solutions were prepared from 11 plant extracts and the staining ability in rat skin tissue was compared with the routinely used Hematoxylin and Eosin (H&E) stain. Punica granulatum, Bixa orellana, Termanalia chebula, Rheum emodi, Rubia tinctorium, Beta vulgaris, Lawsonia inermis, and Tagetes erecta extracts showed more staining intensity. This is the first study to demonstrate the staining ability of Termanalia chebula, Rheum emodi, and Tagetes erecta in biological tissue. Among all plants, Rubia tinctorium and Beta vulgaris showed better staining ability, but lower than H&E. By modifying the concentration, pH, and staining time, we could get better natural stains for histology.
AB - Histopathological diagnosis involves examination of slides stained by chemical stains which are health hazard to researchers, technicians and pathologists. This study screened plant based natural stains for histological applications. About 176 test staining solutions were prepared from 11 plant extracts and the staining ability in rat skin tissue was compared with the routinely used Hematoxylin and Eosin (H&E) stain. Punica granulatum, Bixa orellana, Termanalia chebula, Rheum emodi, Rubia tinctorium, Beta vulgaris, Lawsonia inermis, and Tagetes erecta extracts showed more staining intensity. This is the first study to demonstrate the staining ability of Termanalia chebula, Rheum emodi, and Tagetes erecta in biological tissue. Among all plants, Rubia tinctorium and Beta vulgaris showed better staining ability, but lower than H&E. By modifying the concentration, pH, and staining time, we could get better natural stains for histology.
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U2 - 10.47750/pnr.2022.13.S08.97
DO - 10.47750/pnr.2022.13.S08.97
M3 - Article
AN - SCOPUS:85143358330
SN - 0976-9234
VL - 13
SP - 757
EP - 768
JO - Journal of Pharmaceutical Negative Results
JF - Journal of Pharmaceutical Negative Results
ER -