TY - GEN
T1 - Screening of plant Macaranga peltata for its antioxidant, antimicrobial and cytotoxicity activity
AU - Verma, Meenakshi
AU - Raj, P. Vasanth
AU - Chandrashekhar, Raghu H
AU - Rao, J. Venkata
AU - Udupa, N.
PY - 2009
Y1 - 2009
N2 - The plant Macaranga peltata (Euphorbiaceae) leaves and stem bark was tested for its in vitro antioxidant, antimicrobial and cytotoxicity activity using different methods. In vitro anti oxidant activity Stem Bark extract showed Inhibitory concentration 50 % (IC50) value of 10.13 than the Leaf extract IC50=14.85 for DPPH Assay. Standard Ascorbic acid showed IC50=7.28. For ABTS free radical scavenging activity Leaf extract IC50=7.61 showed better activity than the Stem Bark extract IC50=9.77. Standard anti-oxidant Ascorbic acid showed IC50=11.76. The Nitric Oxide Radical inhibition activity was better shown by Stem Bark extract IC50=573.39 than Leaf extract IC50=>1000. Whereas IC50 value for the standard anti-oxidant Ascorbic acid was 127.16. Scavenging of Superoxide radical by Alkaline DMSO method i.e. NBT Assay showed better activity for Leaf extract IC50=54.12 than Stem Bark extract IC50=55.52. But both Stem Bark and Leaf extract showed very good activity for NBT Assay and their IC50 values were very close to the Standard anti-oxidant Rutin IC50=49.73. In anti microbial activity MIC for Leaf extract was between 62.5μg/ml to 125μg/ml for Escherichia coli, 125μg/ml to 250μg/ml for Pseudomonas aeruginosa, 62.5μg/ml to 125μg/ml for Bacillus subtilis and 31.25μg/ml to 62.5μg/ml for Staphylococcus aureus. Whereas MIC for Stem Bark extract was between 500μg/ml to 1000μg/ml for Escherichia coli, 250μg/ml to 500μg/ml for Pseudomonas aeruginosa, 62.5μg/ml to 125μg/ml for Bacillus subtilis and 62.5μg/ml to 125μg/ml for Staphylococcus aureus. Ciprofloxacin used as standard antibiotic showed MIC between 1.953 μg/ml to7.813 μg/ml for all four bacterial strains. Cytotoxicity of Macaranga peltata Leaf and Stem Bark extracts was tested on Human Liver Cancer cell line HepG2. Four concentrations (1000μg/ml, 500μg/ml, 250μg/ml and 125μg/ml) of both extract were tested for the activity by MTT assay. Both Leaf and Stem Bark extracts showed very good cytotoxicity activity and their CTC50 value was 51.07 μg/ml and 22.32 μg/ml respectively.
AB - The plant Macaranga peltata (Euphorbiaceae) leaves and stem bark was tested for its in vitro antioxidant, antimicrobial and cytotoxicity activity using different methods. In vitro anti oxidant activity Stem Bark extract showed Inhibitory concentration 50 % (IC50) value of 10.13 than the Leaf extract IC50=14.85 for DPPH Assay. Standard Ascorbic acid showed IC50=7.28. For ABTS free radical scavenging activity Leaf extract IC50=7.61 showed better activity than the Stem Bark extract IC50=9.77. Standard anti-oxidant Ascorbic acid showed IC50=11.76. The Nitric Oxide Radical inhibition activity was better shown by Stem Bark extract IC50=573.39 than Leaf extract IC50=>1000. Whereas IC50 value for the standard anti-oxidant Ascorbic acid was 127.16. Scavenging of Superoxide radical by Alkaline DMSO method i.e. NBT Assay showed better activity for Leaf extract IC50=54.12 than Stem Bark extract IC50=55.52. But both Stem Bark and Leaf extract showed very good activity for NBT Assay and their IC50 values were very close to the Standard anti-oxidant Rutin IC50=49.73. In anti microbial activity MIC for Leaf extract was between 62.5μg/ml to 125μg/ml for Escherichia coli, 125μg/ml to 250μg/ml for Pseudomonas aeruginosa, 62.5μg/ml to 125μg/ml for Bacillus subtilis and 31.25μg/ml to 62.5μg/ml for Staphylococcus aureus. Whereas MIC for Stem Bark extract was between 500μg/ml to 1000μg/ml for Escherichia coli, 250μg/ml to 500μg/ml for Pseudomonas aeruginosa, 62.5μg/ml to 125μg/ml for Bacillus subtilis and 62.5μg/ml to 125μg/ml for Staphylococcus aureus. Ciprofloxacin used as standard antibiotic showed MIC between 1.953 μg/ml to7.813 μg/ml for all four bacterial strains. Cytotoxicity of Macaranga peltata Leaf and Stem Bark extracts was tested on Human Liver Cancer cell line HepG2. Four concentrations (1000μg/ml, 500μg/ml, 250μg/ml and 125μg/ml) of both extract were tested for the activity by MTT assay. Both Leaf and Stem Bark extracts showed very good cytotoxicity activity and their CTC50 value was 51.07 μg/ml and 22.32 μg/ml respectively.
UR - http://www.scopus.com/inward/record.url?scp=77950848599&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950848599&partnerID=8YFLogxK
U2 - 10.1109/ICBPE.2009.5384086
DO - 10.1109/ICBPE.2009.5384086
M3 - Conference contribution
AN - SCOPUS:77950848599
SN - 9781424447640
T3 - 2nd International Conference on Biomedical and Pharmaceutical Engineering, ICBPE 2009 - Conference Proceedings
BT - 2nd International Conference on Biomedical and Pharmaceutical Engineering, ICBPE 2009 - Conference Proceedings
T2 - 2nd International Conference on Biomedical and Pharmaceutical Engineering, ICBPE 2009
Y2 - 2 December 2009 through 4 December 2009
ER -
