Selection of cryoprotective agent for freeze drying of valsartan solid lipid nanoparticles

Lalit Kumar, Meka S. Reddy, Ruchi Verma, K. B. Koteshwara

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


The aim of the present study was to select the cryoprotective agent for valsartan solid lipidnanoparticles. The cryoprotective agents were mixed with prepared nanoemulsion and lyophilized for 24h. The cryoprotective agent and its concentration were selected on the basis of particle size, polydispersity index and zeta potential. The stability of prepared nanoparticles (with and without cryoprotective agents)was also determined in respect of particle size and polydispersity index. The cryoprotective agents showed their effect in the following order: mannitol > lactose > sorbitol > maltose > glucose > sucrose > PVP > fructose > starch > dextrose. The particle size, PDI and zeta potential of the nanoformulations containing 10 % mannitol was found to be 403 nm, 0.39 and -19.27 mV, respectively. Six months accelerated stabilitystudy data does not show any significant effect (at p > 005) of temperature on the lyophilized nanoformulations.The present study concludes that the mannitol is a very good cryoprotective agent to retain thesmall particle size and least PDI value of valsartan nanoformulations even at temperatures of 30 and 40°C.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalLatin American Journal of Pharmacy
Issue number2
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery


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