Selective precipitation of haptoglobin and α2-macroglobulin from human serum using Alocasia macrorhiza tuber protein

Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatograpbed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α₂-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α₂-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent α₂-globulins.