TY - JOUR
T1 - Selective precipitation of haptoglobin and α2-macroglobulin from human serum using Alocasia macrorhiza tuber protein
AU - Nayak, B. Shívananda
AU - Ulloor, N. Jagadish
AU - Shívaraj, B.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatograpbed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent α2-globulins.
AB - Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatograpbed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented α2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of α2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent α2-globulins.
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U2 - 10.2174/0929866023408391
DO - 10.2174/0929866023408391
M3 - Article
C2 - 12553858
AN - SCOPUS:0037005351
SN - 0929-8665
VL - 9
SP - 503
EP - 510
JO - Protein and Peptide Letters
JF - Protein and Peptide Letters
IS - 6
ER -