TY - JOUR
T1 - Serum protein profile study of myocardial infarction using a LED induced fluorescence based HPLC system
AU - John, Reena V.
AU - Devasia, Tom
AU - Adigal, Sphurti S.
AU - Lukose, Jijo
AU - Kartha, V. B.
AU - Chidangil, Santhosh
N1 - Funding Information:
Authors are thankful to DST, DST-FIST, DBT Govt. of India, VGST, Govt. of Karnataka and Manipal Academy of Higher Education, Manipal, for the research facilities and Ms. Reena V John is thankful to Manipal Academy of Higher Education for Dr. TMA Pai Ph.D. fellowship and Directorate of Minority fellowship, Govt. of Karnataka, India. Ethics Statement. Ethical clearance for the study is taken from institutional ethical committee (IEC) of Kasturba Hospital, Manipal (IEC 346/2017). Informed consent was taken from the participants before collecting the blood.
Funding Information:
Authors are thankful to DST, DST-FIST, DBT Govt. of India, VGST, Govt. of Karnataka and Manipal Academy of Higher Education, Manipal, for the research facilities and Ms. Reena V John is thankful to Manipal Academy of Higher Education for Dr. TMA Pai Ph.D. fellowship and Directorate of Minority fellowship, Govt. of Karnataka, India.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/2/15
Y1 - 2023/2/15
N2 - Cardiovascular diseases (CVDs) are the major health conditions for high mortality and morbidity in humans. Delay in the diagnosis of CVDs effect patients long and short-term health condition. In -house assembled UV-light emitting diode (LED) based fluorescence detector for high -performance liquid chromatography (HPLC) (HPLC-LED-IF) system is used to record serum chromatograms of three categories of samples namely, before medicated- myocardial infarction (B-MI), after medicated- MI (A-MI), and normal. The sensitivity and performance of HPLC-LED-IF system is estimated using commercial serum proteins. Statistical analysis tools like, descriptive statistics, principal component analysis (PCA), and Match/ No Match test were applied to visualize the variation in three groups of samples. Statistical analysis of the protein profile data showed fairly good discrimination among the three categories. The receiver operating characteristic (ROC) curve also supported the reliability of the method to diagnose MI.
AB - Cardiovascular diseases (CVDs) are the major health conditions for high mortality and morbidity in humans. Delay in the diagnosis of CVDs effect patients long and short-term health condition. In -house assembled UV-light emitting diode (LED) based fluorescence detector for high -performance liquid chromatography (HPLC) (HPLC-LED-IF) system is used to record serum chromatograms of three categories of samples namely, before medicated- myocardial infarction (B-MI), after medicated- MI (A-MI), and normal. The sensitivity and performance of HPLC-LED-IF system is estimated using commercial serum proteins. Statistical analysis tools like, descriptive statistics, principal component analysis (PCA), and Match/ No Match test were applied to visualize the variation in three groups of samples. Statistical analysis of the protein profile data showed fairly good discrimination among the three categories. The receiver operating characteristic (ROC) curve also supported the reliability of the method to diagnose MI.
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U2 - 10.1016/j.jchromb.2023.123616
DO - 10.1016/j.jchromb.2023.123616
M3 - Article
AN - SCOPUS:85148043417
SN - 1570-0232
VL - 1217
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
M1 - 123616
ER -
