TY - JOUR
T1 - Shelf life determination of Khushmanda Rasayana towards scientific evidence for the chemical stability
AU - Ali, Syeda Alisha Md Isha
AU - Gaddam, Niharika
AU - Muddukrishna, Badamane Sathyanarayana
AU - Bhat, Krishnamurthy
AU - Datarkar, Sandeep
AU - Ballal, Murlidhar
AU - Vasantharaju, Surenehalli Gowdra
N1 - Publisher Copyright:
© 2024 Syeda Alisha Md. Isha Ali et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). All Rights Reserved.
PY - 2024/3
Y1 - 2024/3
N2 - Shelf life determination of herbal formulations based on a piece of scientific evidence for a mentioned period and storage conditions defined on the container label becomes paramount for quality. This work is aimed at evaluating the shelf life of Khushmanda Rasayana by reverse phase high performance liquid chromatography. International Council for Harmonisation storage conditions at specific time points were used to determine the marker content in the formulation. Stability studies were conducted at accelerated conditions and long-term conditions at time points (0, 1, 3, 6, 9, and 12 months). The two primary molecular markers used for developing Khushmanda Rasayana’s chemical fingerprint were Catechin and Piperine. Various quality control parameters such as appearance, thin layer chromatography, limit of detection, total ash, acid insoluble ash, alcohol soluble extractive, water soluble extractive, pH, and assay were observed by the Ayurvedic Pharmacopoeia of India. Analysis of the chemical markers over the prescribed period revealed a shelf life of 48.1 months considering the markers Catechin and Piperine. The shelf life estimated indicates the relative stability; this research expands the scope to conduct commercial stability studies in the future.
AB - Shelf life determination of herbal formulations based on a piece of scientific evidence for a mentioned period and storage conditions defined on the container label becomes paramount for quality. This work is aimed at evaluating the shelf life of Khushmanda Rasayana by reverse phase high performance liquid chromatography. International Council for Harmonisation storage conditions at specific time points were used to determine the marker content in the formulation. Stability studies were conducted at accelerated conditions and long-term conditions at time points (0, 1, 3, 6, 9, and 12 months). The two primary molecular markers used for developing Khushmanda Rasayana’s chemical fingerprint were Catechin and Piperine. Various quality control parameters such as appearance, thin layer chromatography, limit of detection, total ash, acid insoluble ash, alcohol soluble extractive, water soluble extractive, pH, and assay were observed by the Ayurvedic Pharmacopoeia of India. Analysis of the chemical markers over the prescribed period revealed a shelf life of 48.1 months considering the markers Catechin and Piperine. The shelf life estimated indicates the relative stability; this research expands the scope to conduct commercial stability studies in the future.
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U2 - 10.7324/JAPS.2024.161815
DO - 10.7324/JAPS.2024.161815
M3 - Article
AN - SCOPUS:85187533344
SN - 2231-3354
VL - 14
SP - 136
EP - 144
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
IS - 3
ER -