TY - JOUR
T1 - Single cell spectroscopy of red blood cells in intravenous crystalloid fluids
AU - N, Mithun
AU - Lukose, Jijo
AU - Mohan, Ganesh
AU - Shastry, Shamee
AU - Chidangil, Santhosh
N1 - Funding Information:
Authors are thankful to DBT, Govt. of India, for the Raman Tweezers facility, DST-FIST, Govt. of. India, for other characterization facility and VGST, Govt. of Karnataka for providing the financial support for the fellows working in the project. Mithun N also acknowledges Manipal Academy of Higher Education for Dr. TMA Pai Doctoral fellowship. Authors also like to acknowledge Vittal Shenoy and the staffs in Blood Bank, Kasturba Medical College, Manipal for their support during this research work.
Publisher Copyright:
© 2021
PY - 2021/8/5
Y1 - 2021/8/5
N2 - Crystalloid fluids, a subset of intravenous (IV) fluid solutions are commonly used in clinical settings. The influence of these fluids on the functions of blood components are least explored. Raman spectroscopy combined with optical trapping has been widely used to evaluate the impact of external stress agents on red blood cells. The present study investigates the impact of commonly used crystalloid fluids on red blood cells in comparison with that of blood plasma using Raman Tweezers spectroscopy. The red blood cells suspended in crystalloid fluids undergo deoxygenation readily than that in blood plasma. In addition, cells in blood plasma were able to withstand laser induced deoxygenation comparatively better than that in crystalloid fluids at higher laser powers. Principle component analysis of the Raman spectral data has clearly demonstrated the discrimination of cells in plasma with that of crystalloid fluids demonstrating the effect of external induced stress on RBCs.
AB - Crystalloid fluids, a subset of intravenous (IV) fluid solutions are commonly used in clinical settings. The influence of these fluids on the functions of blood components are least explored. Raman spectroscopy combined with optical trapping has been widely used to evaluate the impact of external stress agents on red blood cells. The present study investigates the impact of commonly used crystalloid fluids on red blood cells in comparison with that of blood plasma using Raman Tweezers spectroscopy. The red blood cells suspended in crystalloid fluids undergo deoxygenation readily than that in blood plasma. In addition, cells in blood plasma were able to withstand laser induced deoxygenation comparatively better than that in crystalloid fluids at higher laser powers. Principle component analysis of the Raman spectral data has clearly demonstrated the discrimination of cells in plasma with that of crystalloid fluids demonstrating the effect of external induced stress on RBCs.
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U2 - 10.1016/j.saa.2021.119726
DO - 10.1016/j.saa.2021.119726
M3 - Article
AN - SCOPUS:85103976877
SN - 1386-1425
VL - 257
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
M1 - 119726
ER -