TY - JOUR
T1 - Small interfering RNAs (siRNAs) based gene silencing strategies for the treatment of glaucoma
T2 - Recent advancements and future perspectives
AU - Naik, Santoshi
AU - Shreya, Ajjappla Basavaraj
AU - Raychaudhuri, Ruchira
AU - Pandey, Abhijeet
AU - Lewis, Shaila A.
AU - Hazarika, Manali
AU - Bhandary, Sulatha V.
AU - Rao, Bola Sadashiva Satish
AU - Mutalik, Srinivas
N1 - Funding Information:
The authors are thankful to – i) Department of Science and Technology (DST), Government of India, New Delhi for DST-INSPIRE Junior Research Fellowship to Ms. Santoshi Naik, ii) Council for Scientific and Industrial Research (CSIR), Government of India, New Delhi for Junior Research Fellowship to Ms. Ruchira Raychaudhuri, iii) Science and Engineering Research Board (SERB), Government of India, New Delhi for Research Fellowship to Ms. A B Shreya and iv) Manipal Academy of Higher Education, Manipal for Postdoctoral Research Fellowship to Dr. Abhijeet Pandey. The authors are grateful to all the above funding agencies ( DST , CSIR , and SERB ) and Manipal Academy of Higher Education, Manipal for providing financial and infrastructural support for the research activities.
Publisher Copyright:
© 2020
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - RNA-interference-based mechanisms, especially the use of small interfering RNAs (siRNAs), have been under investigation for the treatment of several ailments and have shown promising results for ocular diseases including glaucoma. The eye, being a confined compartment, serves as a good target for the delivery of siRNAs. This review focuses on siRNA-based strategies for gene silencing to treat glaucoma. We have discussed the ocular structures and barriers to gene therapy (tear film, corneal, conjunctival, vitreous, and blood ocular barriers), methods of administration for ocular gene delivery (topical instillation, periocular, intracameral, intravitreal, subretinal, and suprachoroidal routes) and various viral and non-viral vectors in siRNA-based therapy for glaucoma. The components and mechanism of siRNA-based gene silencing have been mentioned briefly followed by the basic strategies and challenges faced during siRNA therapeutics development. We have emphasized different therapeutic targets for glaucoma which have been under research by scientists and the current siRNA-based drugs used in glaucoma treatment. We also mention briefly strategies for siRNA-based treatment after glaucoma surgery.
AB - RNA-interference-based mechanisms, especially the use of small interfering RNAs (siRNAs), have been under investigation for the treatment of several ailments and have shown promising results for ocular diseases including glaucoma. The eye, being a confined compartment, serves as a good target for the delivery of siRNAs. This review focuses on siRNA-based strategies for gene silencing to treat glaucoma. We have discussed the ocular structures and barriers to gene therapy (tear film, corneal, conjunctival, vitreous, and blood ocular barriers), methods of administration for ocular gene delivery (topical instillation, periocular, intracameral, intravitreal, subretinal, and suprachoroidal routes) and various viral and non-viral vectors in siRNA-based therapy for glaucoma. The components and mechanism of siRNA-based gene silencing have been mentioned briefly followed by the basic strategies and challenges faced during siRNA therapeutics development. We have emphasized different therapeutic targets for glaucoma which have been under research by scientists and the current siRNA-based drugs used in glaucoma treatment. We also mention briefly strategies for siRNA-based treatment after glaucoma surgery.
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U2 - 10.1016/j.lfs.2020.118712
DO - 10.1016/j.lfs.2020.118712
M3 - Review article
C2 - 33159955
AN - SCOPUS:85095835334
SN - 0024-3205
VL - 264
JO - Life Sciences
JF - Life Sciences
M1 - 118712
ER -