Abstract
A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of Tramadol hydrochloride in pure and pharmaceutical dosage form. Tramadol hydrochloride showed strong native fluorescence in 0.025 M sulphuric acid having excitation at 220.0 nm and emission at 297±5 nm. All parameters viz; effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.025 to 0.400 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The limit of detection and limit of quantification were found to be 0.0053 mcg/mL and 0.0170 mcg/mL srespectively. The percentage recovery was found to be 99.42%.
| Original language | English |
|---|---|
| Pages (from-to) | 32-34 |
| Number of pages | 3 |
| Journal | Indian Drugs |
| Volume | 46 |
| Issue number | 8 |
| Publication status | Published - 01-08-2009 |
All Science Journal Classification (ASJC) codes
- Pharmacology
- Pharmaceutical Science
- Drug Discovery