Male factor infertility remains a significant problem affecting approximately one couple in six. The genetic integrity of a male gamete is necessary for the normal fertilization and development of the offspring. It has been shown that any insult to sperm DNA may have adverse influence on the reproductive outcome. Most human ejaculates contain a subpopulation of sperm with DNA damage, also referred to as DNA fragmentation, in the form of double- or single-strand breaks which have been induced in the DNA prior to or following ejaculation. The routine semen analysis does not reveal the genetic integrity of the sperm; hence, special techniques are employed for the detection of sperm DNA fragmentation. This chapter discusses about the causes of sperm DNA damage and various techniques employed to detect DNA fragmentation in spermatozoa.
|Title of host publication||Male Infertility|
|Subtitle of host publication||A Clinical Approach|
|Number of pages||11|
|Publication status||Published - 01-01-2016|
All Science Journal Classification (ASJC) codes
- Health Professions(all)