Sperm DNA damage: Causes and laboratory detection

Satish Kumar Adiga; Guruprasad Kalthur

doi:10.1007/978-81-322-3604-7_10

Sperm DNA damage: Causes and laboratory detection

Satish Kumar Adiga^*, Guruprasad Kalthur

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Male factor infertility remains a significant problem affecting approximately one couple in six. The genetic integrity of a male gamete is necessary for the normal fertilization and development of the offspring. It has been shown that any insult to sperm DNA may have adverse influence on the reproductive outcome. Most human ejaculates contain a subpopulation of sperm with DNA damage, also referred to as DNA fragmentation, in the form of double- or single-strand breaks which have been induced in the DNA prior to or following ejaculation. The routine semen analysis does not reveal the genetic integrity of the sperm; hence, special techniques are employed for the detection of sperm DNA fragmentation. This chapter discusses about the causes of sperm DNA damage and various techniques employed to detect DNA fragmentation in spermatozoa.

Original language	English
Title of host publication	Male Infertility
Subtitle of host publication	A Clinical Approach
Publisher	Springer India
Pages	155-165
Number of pages	11
ISBN (Electronic)	9788132236047
ISBN (Print)	9788132236023
DOIs	https://doi.org/10.1007/978-81-322-3604-7_10
Publication status	Published - 01-01-2016

All Science Journal Classification (ASJC) codes

General Health Professions
General Medicine

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10.1007/978-81-322-3604-7_10

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Sperm DNA damage: Causes and laboratory detection

Abstract

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