TY - JOUR
T1 - Statistical optimisation of polyhydroxyalkanoate production in Bacillus endophyticus using sucrose as sole source of carbon
AU - Geethu, M.
AU - Chandrashekar, H. Raghu
AU - Divyashree, M. S.
N1 - Funding Information:
Open access funding provided by Manipal Academy of Higher Education, Manipal. This study was supported by The Department of Biotechnology (DBT), Government of India, for sponsoring the work (PR 18430/BIC/101/703/2016).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57% which was in well agreement with the given predicted value provided by response surface methodology model yield of 47.02%. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9. This is the first report highlighting 49% of polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and 1HNMR analysis confirmed the polymer produced.
AB - Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57% which was in well agreement with the given predicted value provided by response surface methodology model yield of 47.02%. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9. This is the first report highlighting 49% of polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and 1HNMR analysis confirmed the polymer produced.
UR - http://www.scopus.com/inward/record.url?scp=85115302904&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85115302904&partnerID=8YFLogxK
U2 - 10.1007/s00203-021-02554-6
DO - 10.1007/s00203-021-02554-6
M3 - Article
AN - SCOPUS:85115302904
SN - 0302-8933
VL - 203
SP - 5993
EP - 6005
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 10
ER -