Studies on γ-glutamyl transpeptidase of human urine

K. Rambabu; T.N. Pattabiraman

doi:10.1016/0009-8981(76)90170-4

Studies on γ-glutamyl transpeptidase of human urine

K. Rambabu, T.N. Pattabiraman

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A simple and rapid method suitable for clinical laboratories, for the removal of the inhibitors in urine of γ-glutamyl transpeptidase activity, using an ion-retardation resin, is described. Gel chromatography of urine resulted in appreciable loss of the enzyme activity. Freezing at -5°C and thawing, did not affect the activity of the enzyme. Dimethyl sulfoxide and polyvinyl pyrrolidone up to a concentration of 20% did not have any effect on the transpeptidase activity. Urea, creatinine and NaCl at levels found in normal urine did not inhibit the transpeptidase. © 1976.

Original language	English
Pages (from-to)	251-255
Number of pages	5
Journal	Clinica Chimica Acta
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(76)90170-4
Publication status	Published - 1976
Externally published	Yes

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abstract = "A simple and rapid method suitable for clinical laboratories, for the removal of the inhibitors in urine of γ-glutamyl transpeptidase activity, using an ion-retardation resin, is described. Gel chromatography of urine resulted in appreciable loss of the enzyme activity. Freezing at -5°C and thawing, did not affect the activity of the enzyme. Dimethyl sulfoxide and polyvinyl pyrrolidone up to a concentration of 20\% did not have any effect on the transpeptidase activity. Urea, creatinine and NaCl at levels found in normal urine did not inhibit the transpeptidase. {\textcopyright} 1976.",

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AU - Rambabu, K.

AU - Pattabiraman, T.N.

N1 - cited By 14

PY - 1976

Y1 - 1976

N2 - A simple and rapid method suitable for clinical laboratories, for the removal of the inhibitors in urine of γ-glutamyl transpeptidase activity, using an ion-retardation resin, is described. Gel chromatography of urine resulted in appreciable loss of the enzyme activity. Freezing at -5°C and thawing, did not affect the activity of the enzyme. Dimethyl sulfoxide and polyvinyl pyrrolidone up to a concentration of 20% did not have any effect on the transpeptidase activity. Urea, creatinine and NaCl at levels found in normal urine did not inhibit the transpeptidase. © 1976.

AB - A simple and rapid method suitable for clinical laboratories, for the removal of the inhibitors in urine of γ-glutamyl transpeptidase activity, using an ion-retardation resin, is described. Gel chromatography of urine resulted in appreciable loss of the enzyme activity. Freezing at -5°C and thawing, did not affect the activity of the enzyme. Dimethyl sulfoxide and polyvinyl pyrrolidone up to a concentration of 20% did not have any effect on the transpeptidase activity. Urea, creatinine and NaCl at levels found in normal urine did not inhibit the transpeptidase. © 1976.

U2 - 10.1016/0009-8981(76)90170-4

DO - 10.1016/0009-8981(76)90170-4

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VL - 73

SP - 251

EP - 255

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 2

ER -

Studies on γ-glutamyl transpeptidase of human urine

Abstract

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