TY - JOUR
T1 - Studies on the antioxidant activities of Desmodium gangeticum
AU - Govindarajan, R.
AU - Rastogi, S.
AU - Vijayakumar, M.
AU - Shirwaikar, A.
AU - Rawat, A.K.S.
AU - Mehrotra, S.
AU - Pushpangadan, P.
N1 - Cited By :198
Export Date: 10 November 2017
CODEN: BPBLE
Correspondence Address: Mehrotra, S.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: [email protected]
Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; 5 mercapto 2 nitrobenzoic acid, 15139-21-6; ferrous ion, 15438-31-0; hypochlorous acid, 7790-92-3; nitric oxide, 10102-43-9; nitroprusside sodium, 14402-89-2, 15078-28-1; Antioxidants; Free Radical Scavengers; Plant Extracts
References: Frei, B., (1999) FASEB J., 13, pp. 963-964; Zupko, I., Hohmann, J., Redei, D., Falkay, G., Janicsak, G., Mathe, I., (2001) Planta Med., 67, pp. 366-368; Valentao, P., Fernandes, E., Carvalho, F., Andrad, P.B., Seabra, R.M., Bostos, M.L., (2001) J. Agric. Food. Chem., 49, pp. 3476-3479; Haraguchi, H., Saitao, T., Ishikawa, H., Date, H., Kataoka, S., Tamura, Y., Mizutani, K., (1996) Planta Med., 62, pp. 217-221; Soares, J.R., Dinis, T.C.P., Cunha, A.P., Almeida, L., (1997) Free Rad. Res., 26, pp. 469-478; Chopra, R.N., Nayar, S.L., Chopra, I.C., (1956) Glossary of Indian Medicinal Plants, p. 94. , Council of Scientific and Industrial Research, New Delhi; Jabbar, S., Khan, M.T., Choudhuri, M.S., (2001) Pharmazie, 56, pp. 506-508; Iwu, M.M., Jackson, J.E., Tally, J.D., Klayman, D.L., (1992) Planta Med., 58, pp. 436-441; Ghosh, D., Anandkumar, A., (1981) Indian J. Pharmacol., 15, pp. 391-402; Ghosal, S., Bhattacharya, S.K., (1972) Planta Med., 22, pp. 434-440; Purushothman, K.K., Kishore, V.M., Narayanaswamy, V., (1971) J. Chem. Soc. (C), 1971, pp. 2420-2422; Sreejayan, N., Rao, M.N.A., (1996) Drug Research, 46, pp. 169-171; Preito, P., Pineda, M., Aguilar, M., (1999) Anal. Biochem., 269, pp. 337-341; Sreejayan, N., Rao, M.N.A., (1997) J. Pharm. Pharmacol., 49, pp. 105-107; Paya, M., Halliwell, B., Hoult, J.R.S., (1992) Biochem. Pharmacol., 44, pp. 205-214; Ching, T.L., De Jong, J., Bast, A., (1994) Anal. Biochem., 218, pp. 377-381; Valentao, P., Fernndes, E., Canvalho, F., Andrade, P.B., Seabra, R.M., Bastos, M.L., (2002) Biol. Pharm. Bull., 25, pp. 1324-1327; Harris, G.M., Livingstone, S.E., (1964) Bidentate Chelates in Chelating Agents and Metal Chelates, 1, p. 95. , ed. by Dwyer F. P., Mellor D. P., Academic Press, New York; Sinha, A.K., (1972) Anal. Biochem., 47, pp. 389-394; Okhawa, H., Ohishi, N., Yagi, K., (1979) Anal. Biochem., 95, pp. 351-355; Tripathi, Y.B., Sharma, M., (1998) Indian J. Biochem. Biophys., 35, pp. 313-316; Shinmoto, H., Dosako, S., Nakajima, I., (1992) Biosci. Biotech. Biochem., 56, pp. 2079-2080; Bhattacharya, A., Chatterjee, A., Ghosal, S., Bhattacharya, S.K., (1999) Indian J. Exp. Biol., 37, pp. 676-680; Miller, M.J.S., Sadowska-Krowicka, H., Chotinaruemol, S., Kakkis, J.L., Clark, D.A., (1992) J. Pharmacol. Exp. Therapeutics, 264, pp. 11-16; Aruoma, O.L., Halliwell, B., Hoey, B.M., Butler, J., (1989) Free Rad. Biol. Med., 6, pp. 593-597; Halliwell, B., Gutteridge, J.M.C., (1993) Free Radicals in Biology and Medicine, pp. 79-86. , Clarendon, Oxford
PY - 2003
Y1 - 2003
N2 - Desmodium gangeticum is herbal species which is widely used in the indigenous system of medicine and is reported to contain flavone and isoflavanoid glycosides. In view of its wide use and it's chemical composition, this study was aimed at examining the antioxidant activity of the extract of D. gangeticum. The extract was studied for diphenyl picryl hydrazyl (DPPH), nitric oxide, ferryl-bipyridyl and hypochlorous acid scavenging activity along with lipid peroxidation. Nitric oxide was generated using sodium nitroprusside and was studied using Griess reagent. In order to study the iron chelating capacity of the extract, the percentage ferryl-bipyridyl inhibition was studied. Hypochlorous acid scavenging activity was tested by measuring the inhibition of 5-thio-2-nitrobenzoic acid oxidation. The extract was also studied for lipid peroxidation assay by thiobarbituric acid-reactive substances (TBARS) method using rat brain homogenate. The results indicate that D. gangeticum extract has potent antioxidant activity. © 2003 Pharmaceutical Society of Japan.
AB - Desmodium gangeticum is herbal species which is widely used in the indigenous system of medicine and is reported to contain flavone and isoflavanoid glycosides. In view of its wide use and it's chemical composition, this study was aimed at examining the antioxidant activity of the extract of D. gangeticum. The extract was studied for diphenyl picryl hydrazyl (DPPH), nitric oxide, ferryl-bipyridyl and hypochlorous acid scavenging activity along with lipid peroxidation. Nitric oxide was generated using sodium nitroprusside and was studied using Griess reagent. In order to study the iron chelating capacity of the extract, the percentage ferryl-bipyridyl inhibition was studied. Hypochlorous acid scavenging activity was tested by measuring the inhibition of 5-thio-2-nitrobenzoic acid oxidation. The extract was also studied for lipid peroxidation assay by thiobarbituric acid-reactive substances (TBARS) method using rat brain homogenate. The results indicate that D. gangeticum extract has potent antioxidant activity. © 2003 Pharmaceutical Society of Japan.
U2 - 10.1248/bpb.26.1424
DO - 10.1248/bpb.26.1424
M3 - Article
SN - 0918-6158
VL - 26
SP - 1424
EP - 1427
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 10
ER -