Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells

S. A. Salmataj; Shobha Kamath; null Ramachandramurty

Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells

S. A. Salmataj, Shobha Kamath, Ramachandramurty

Research output: Contribution to journal › Article › peer-review

Abstract

Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO₂(As III) and Na₃AsO₄(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC₅₀) of NaAsO₂(12mM) and Na₃AsO₄(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential (Øm). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10% of IC₅₀ value) in IEC-6 cells.

Original language	English
Pages (from-to)	603-610
Number of pages	8
Journal	Asian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume	20
Issue number	2
Publication status	Published - 01-01-2018

All Science Journal Classification (ASJC) codes

Biotechnology
Microbiology
Applied Microbiology and Biotechnology
Environmental Science(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells",

abstract = "Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO2(As III) and Na3AsO4(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC50) of NaAsO2(12mM) and Na3AsO4(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential ({\O}m). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10% of IC50 value) in IEC-6 cells.",

author = "Salmataj, {S. A.} and Shobha Kamath and Ramachandramurty",

year = "2018",

month = jan,

day = "1",

language = "English",

volume = "20",

pages = "603--610",

journal = "Asian Journal of Microbiology, Biotechnology and Environmental Sciences",

issn = "0972-3005",

publisher = "Global Publications",

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TY - JOUR

T1 - Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells

AU - Salmataj, S. A.

AU - Kamath, Shobha

AU - Ramachandramurty, null

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO2(As III) and Na3AsO4(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC50) of NaAsO2(12mM) and Na3AsO4(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential (Øm). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10% of IC50 value) in IEC-6 cells.

AB - Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO2(As III) and Na3AsO4(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC50) of NaAsO2(12mM) and Na3AsO4(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential (Øm). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10% of IC50 value) in IEC-6 cells.

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UR - http://www.scopus.com/inward/citedby.url?scp=85048970638&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85048970638

SN - 0972-3005

VL - 20

SP - 603

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JO - Asian Journal of Microbiology, Biotechnology and Environmental Sciences

JF - Asian Journal of Microbiology, Biotechnology and Environmental Sciences

IS - 2

ER -

Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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