TY - JOUR
T1 - Susceptibility of Candida albicans and Enterococcus faecalis to Chitosan, Chlorhexidine gluconate and their combination in vitro
AU - Ballal, N.V.
AU - Kundabala, M.
AU - Bhat, K.S.
AU - Acharya, S.
AU - Ballal, M.
AU - Kumar, R.
AU - Prakash, P.Y.
N1 - Cited By :17
Export Date: 10 November 2017
Correspondence Address: Ballal, N.V.; Manipal College of Dental Sciences, Manipal-576 104, Karnataka, India; email: [email protected]
Chemicals/CAS: chitosan, 9012-76-4; chlorhexidine, 3697-42-5, 55-56-1; Anti-Infective Agents, Local; Biocompatible Materials; Chitosan, 9012-76-4; Chlorhexidine, 55-56-1; Delayed-Action Preparations; Drug Combinations; Gels
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IV polycarbophil and chitosan are potent enhancers of peptide transport across intestinal mucosae in vitro (1997) J Control Release, 45, pp. 15-23; Schipper, N.G.M., Varum, K.M., Artursson, P., Chitosan as absorption enhancers for poorly absorbable drugs. 1: inuence of molecular weight and degree of acetylation on drug transport across human epithelial cells (CaCo-2) cells (1996) Pharm Res, 13, pp. 1686-1692; Singla, A.K., Chawla, M., Chitosan: some pharmaceutical and biological aspects-an update (2001) J Pharm Pharmacol, 53, pp. 1047-1067; Park, J.S., Choi, S.H., Moon, I.S., Cho, K.S., Chai, J.K., Kim, C.K., Eight week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs (2003) J Clin Periodontol, 30, pp. 443-453; Senel, S., Ikinci, G., Kas, S., Youse Rad, A., Sargon, M.F., Hincal, A.A., Chitosan films and hydrogels of chlorhexidine gluconate for oral mucosal delivery (2000) Int J Pharm, 193, pp. 197-203; Greenstein, G., Berman, C., Jaffin, R., Chlorhexidine. An adjunct to periodontal therapy (1986) J Periodontol, 57, pp. 370-376; Gomes, B.P.F.A., Ferraz, C.C.R., Vianna, M.E., Berber, V.B., Teixeira, F.R., Souza-Filho, F.J., In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis (2001) Int Endod J, 34, pp. 424-428; Staroniewiez, Z., Ramisz, A., Wojtosz-Pajak, A., Brzeski, M.M., (1994) Studies on antibacterial and antifungal activity of chitosan, pp. 374-377. , Karnicki ZS, Brzeski MM, Bykonski PJ, Wojtasz-Pajak A, eds. Chitin world. Wissenschoft, GmbH; Germany: Wirtschaftverlog NW-Verlag furneve
PY - 2009
Y1 - 2009
N2 - The aim of the present study was to analyse the sustain release of Chlorhexidine with Chitosan and to investigate the antimicrobial activity of 2% Chlorhexidine gel, 2% Chitosan gel and their combination against Candida albicans and Enterococcus faecalis. Sustain release of Chlorhexidine with Chitosan was determined using UV spectrophotometer. Then the inoculae of these organisms were used to make the lawn culture on sabouraud's dextrose agar and blood agar plates. Wells were prepared in these lawn cultures and filled with 2% Chlorhexidine gel, 2% Chitosan gel and their combination. The agar plates were incubated overnight at 37°C and the zone of inhibition was examined after 48 h. Release of Chlorhexidine with Chitosan was better than plain Chlorhexidine release. Combination of Chlorhexidine and Chitosan showed maximum inhibitory zone for C. albicans (25.2 mm) and E. faecalis (26.0 mm). Plain Chlorhexidine gel showed intermediate inhibitory zone for C. albicans (20.6 mm) and E. faecalis (21.4 mm) and plain Chitosan gel showed minimum inhibitory zone for C. albicans (16.6 mm) and E. faecalis (11.0 mm). Carbopol which served as control did not have any antimicrobial effect. The present study suggests that 2% Chlorhexidine gel in combination with 2% Chitosan gel has the highest antimicrobial effect against C. albicans and E. faecalis compared with 2% Chlorhexidine gel or 2% Chitosan gel alone. © 2008 The Authors. Journal compilation © 2008 Australian Society of Endodontology.
AB - The aim of the present study was to analyse the sustain release of Chlorhexidine with Chitosan and to investigate the antimicrobial activity of 2% Chlorhexidine gel, 2% Chitosan gel and their combination against Candida albicans and Enterococcus faecalis. Sustain release of Chlorhexidine with Chitosan was determined using UV spectrophotometer. Then the inoculae of these organisms were used to make the lawn culture on sabouraud's dextrose agar and blood agar plates. Wells were prepared in these lawn cultures and filled with 2% Chlorhexidine gel, 2% Chitosan gel and their combination. The agar plates were incubated overnight at 37°C and the zone of inhibition was examined after 48 h. Release of Chlorhexidine with Chitosan was better than plain Chlorhexidine release. Combination of Chlorhexidine and Chitosan showed maximum inhibitory zone for C. albicans (25.2 mm) and E. faecalis (26.0 mm). Plain Chlorhexidine gel showed intermediate inhibitory zone for C. albicans (20.6 mm) and E. faecalis (21.4 mm) and plain Chitosan gel showed minimum inhibitory zone for C. albicans (16.6 mm) and E. faecalis (11.0 mm). Carbopol which served as control did not have any antimicrobial effect. The present study suggests that 2% Chlorhexidine gel in combination with 2% Chitosan gel has the highest antimicrobial effect against C. albicans and E. faecalis compared with 2% Chlorhexidine gel or 2% Chitosan gel alone. © 2008 The Authors. Journal compilation © 2008 Australian Society of Endodontology.
U2 - 10.1111/j.1747-4477.2008.00126.x
DO - 10.1111/j.1747-4477.2008.00126.x
M3 - Article
SN - 1329-1947
VL - 35
SP - 29
EP - 33
JO - Australian Endodontic Journal
JF - Australian Endodontic Journal
IS - 1
ER -