Synthesis of phyto-functionalized nano hematite for lung cancer suppressive activity and paracetamol sensing by electrochemical studies

The present research aims to synthesize hematite nanoparticles (IPC NPs) from Pouteria campechiana extract-based reducing agent. The influence of fuel load on the size and shape of the NPs was studied on varying the volume of the extract utilizing Solution Combustion Synthesis. The IPC NPs were characterized by various microscopic and analytical tools. The synthesized biogenic NPs exhibited enhanced electrochemical activity with the highest proton diffusion coefficient (7.67 ×10⁻⁶ cm²s⁻¹). Fabrication of NPs into stable working electrodes for sensing of Paracetamol, with a concentration range from 1 to 6 mM was studied. The DPPH scavenging activity of 87.38% was recorded with an IC₅₀ of 50.88 μg/mL. Additionally, the NPs also showed a dose-dependent α- amylase inhibition activity. The Human Red Blood Cells Stabilization assay carried out has evinced the haemocompatibility of hematite NPs with a % stabilization of 84.95. The in-vitro cytotoxicity study manifested the A549 lung cancer suppressive activity of IPC NPs with a cell viability of 33.39% at their higher concentration. To the best of our knowledge, no literature is available on size-controlled hematite nanoparticle synthesis via Solution Combustion Synthesis on the variation of Pouteria campechiana extract and its effect on biomedical and Paracetamol sensing applications.