TY - JOUR
T1 - The in vitro effect of benzo[a]pyrene on human sperm hyperactivation and acrosome reaction
AU - Mukhopadhyay, Dyutiman
AU - Nandi, Parag
AU - Varghese, Alex C.
AU - Gutgutia, Rohit
AU - Banerjee, Samir
AU - Bhattacharyya, Asok K.
PY - 2010/7
Y1 - 2010/7
N2 - Objective: To evaluate the in vitro effect of benzo[a]pyrene on sperm hyperactivation and acrosome status in normozoospermic semen samples of nonsmokers analyzed by computer-assisted semen analysis (CASA). Design: Experimental in vitro study. Setting: Andrology laboratory. Patient(s): Thirteen proven fertile, normozoospermic, and nonsmoking men. Intervention(s): Spermatozoa were washed free of seminal plasma and were treated with different concentrations of benzo[a]pyrene and compared with controls treated with medium alone. The benzo[a]pyrene concentrations were: 100, 50, 25, and 12.5 μg/mL. Main Outcome Measure(s): Effect of varying concentrations of benzo[a]pyrene on sperm hyperactivation and acrosomal reaction. Result(s): A statistically significant increase in sperm hyperactivation was observed in presence of benzo[a]pyrene at concentrations of ≥50 μg/mL. The result of the acrosome halo test showed that concentrations of benzo[a]pyrene ≥25 μg/mL statistically significantly decreased the percentage of halo formation, indicating an inappropriate (false) acrosome reaction. Conclusion(s): Benzo[a]pyrene statistically significantly affected sperm functional competence as evidenced by increased hyperactivation as well as premature acrosomal reaction.
AB - Objective: To evaluate the in vitro effect of benzo[a]pyrene on sperm hyperactivation and acrosome status in normozoospermic semen samples of nonsmokers analyzed by computer-assisted semen analysis (CASA). Design: Experimental in vitro study. Setting: Andrology laboratory. Patient(s): Thirteen proven fertile, normozoospermic, and nonsmoking men. Intervention(s): Spermatozoa were washed free of seminal plasma and were treated with different concentrations of benzo[a]pyrene and compared with controls treated with medium alone. The benzo[a]pyrene concentrations were: 100, 50, 25, and 12.5 μg/mL. Main Outcome Measure(s): Effect of varying concentrations of benzo[a]pyrene on sperm hyperactivation and acrosomal reaction. Result(s): A statistically significant increase in sperm hyperactivation was observed in presence of benzo[a]pyrene at concentrations of ≥50 μg/mL. The result of the acrosome halo test showed that concentrations of benzo[a]pyrene ≥25 μg/mL statistically significantly decreased the percentage of halo formation, indicating an inappropriate (false) acrosome reaction. Conclusion(s): Benzo[a]pyrene statistically significantly affected sperm functional competence as evidenced by increased hyperactivation as well as premature acrosomal reaction.
UR - https://www.scopus.com/pages/publications/77953719349
UR - https://www.scopus.com/pages/publications/77953719349#tab=citedBy
U2 - 10.1016/j.fertnstert.2009.02.031
DO - 10.1016/j.fertnstert.2009.02.031
M3 - Article
C2 - 19324334
AN - SCOPUS:77953719349
SN - 0015-0282
VL - 94
SP - 595
EP - 598
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 2
ER -