TY - JOUR
T1 - The ubiquitin-like protein Hub1/UBL-5 functions in pre-mRNA splicing in Caenorhabditis elegans
AU - Kolathur, Kiran Kumar
AU - Sharma, Pallavi
AU - Kadam, Nagesh Y.
AU - Shahi, Navneet
AU - Nishitha, Ane
AU - Babu, Kavita
AU - Mishra, Shravan Kumar
N1 - Funding Information:
We thank Genotypic Technology Private Limited, Bengaluru, and Ms Parameswari Behera for the Microarray processing and data analysis. The C. elegans strains were provided by CGC, funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). The authors thank Ankit Negi for routine help and Karan Chaudhary for helping with the microarray probe design. The work was supported by the DBT/Welcome Trust India Alliance Fellowship/Grants [grant number IA/I/18/2/504020 awarded to SKM and grant numbers IA/I/12/1/500516 and IA/S/19/2/504649 awarded to KB]. AN thanks DST-INSPIRE for providing the fellowship. Research in the SKM laboratory was supported by the Ministry of Human Resource and Development (MHRD), Department of Science and Technology (DST), Government of India, and the Max Planck Society, Germany. KKK and PS received a graduate fellowship from the Indian Council for Medical Research (ICMR), Government of India, and NYK and NS received scholarships from the Council of Scientific and Industrial Research (CSIR)/University Grants Commission (UGC). KB is also funded by DBT grants BT/PR24038/BRB/10/1693/2018 and BT/HRD-NBA-NWB/38/2019-20, and the Ministry of Education grant MoE/STARS-1/454, which part funded this study.
Funding Information:
We thank Genotypic Technology Private Limited, Bengaluru, and Ms Parameswari Behera for the Microarray processing and data analysis. The strains were provided by CGC, funded by the NIH Office of Research Infrastructure Programs (P40 OD010440). The authors thank Ankit Negi for routine help and Karan Chaudhary for helping with the microarray probe design. The work was supported by the DBT/Welcome Trust India Alliance Fellowship/Grants [grant number IA/I/18/2/504020 awarded to SKM and grant numbers IA/I/12/1/500516 and IA/S/19/2/504649 awarded to KB]. AN thanks DST‐INSPIRE for providing the fellowship. Research in the SKM laboratory was supported by the Ministry of Human Resource and Development (MHRD), Department of Science and Technology (DST), Government of India, and the Max Planck Society, Germany. KKK and PS received a graduate fellowship from the Indian Council for Medical Research (ICMR), Government of India, and NYK and NS received scholarships from the Council of Scientific and Industrial Research (CSIR)/University Grants Commission (UGC). KB is also funded by DBT grants BT/PR24038/BRB/10/1693/2018 and BT/HRD‐NBA‐NWB/38/2019‐20, and the Ministry of Education grant MoE/STARS‐1/454, which part funded this study. C. elegans
Publisher Copyright:
© 2022 Federation of European Biochemical Societies.
PY - 2023/2
Y1 - 2023/2
N2 - The ubiquitin-like protein Hub1/UBL-5 associates with proteins non-covalently. Hub1 promotes alternative splicing and splicing of precursor mRNAs with weak introns in yeast and mammalian cells; however, its splicing function has remained elusive in multicellular organisms. Here, we demonstrate the splicing function of Hub1/UBL-5 in the free-living nematode Caenorhabditis elegans. Hub1/UBL-5 binds to the HIND-containing splicing factors Snu66/SART-1 and PRP-38 and associates with other spliceosomal proteins. C. elegans hub1/ubl-5 mutants die at the Larval 3 stage and show splicing defects for selected targets, similar to the mutants in yeast and mammalian cells. UBL-5 complemented growth and splicing defects in Schizosaccharomyces pombe hub1 mutants, confirming its functional conservation. Thus, UBL-5 is important for C. elegans development and plays a conserved pre-mRNA splicing function.
AB - The ubiquitin-like protein Hub1/UBL-5 associates with proteins non-covalently. Hub1 promotes alternative splicing and splicing of precursor mRNAs with weak introns in yeast and mammalian cells; however, its splicing function has remained elusive in multicellular organisms. Here, we demonstrate the splicing function of Hub1/UBL-5 in the free-living nematode Caenorhabditis elegans. Hub1/UBL-5 binds to the HIND-containing splicing factors Snu66/SART-1 and PRP-38 and associates with other spliceosomal proteins. C. elegans hub1/ubl-5 mutants die at the Larval 3 stage and show splicing defects for selected targets, similar to the mutants in yeast and mammalian cells. UBL-5 complemented growth and splicing defects in Schizosaccharomyces pombe hub1 mutants, confirming its functional conservation. Thus, UBL-5 is important for C. elegans development and plays a conserved pre-mRNA splicing function.
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UR - http://www.scopus.com/inward/citedby.url?scp=85144266537&partnerID=8YFLogxK
U2 - 10.1002/1873-3468.14555
DO - 10.1002/1873-3468.14555
M3 - Article
C2 - 36480405
AN - SCOPUS:85144266537
SN - 0014-5793
VL - 597
SP - 448
EP - 457
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -