TY - JOUR
T1 - Total phenolic content and free radical scavenging activity of Salacia oblonga and Gymnema Sylvestre
T2 - An in vitro comparative study
AU - Uday, Shivani
AU - Vangalapati, Bheemesh
AU - Manjrekar, Poornima A.
AU - Hegde, Anupama
PY - 2015/1/1
Y1 - 2015/1/1
N2 - The over production of free radicals is involved in triggering many diseases such as diabetes, cancer, rheumatoid arthritis, cirrhosis and arteriosclerosis. Antioxidants derived from plant sources offer resistance against the oxidative stress by way of their free radicals scavenging & lipid peroxidation inhibitory actions. Present study was designed to compare the free radical scavenging activity in relation to the total phenolic content of G. sylvestre & S. oblonga. Total phenolic content of the extracts was determined by using Folin-Ciocalteu method following a slightly modified method of Singleton by comparing with standard Gallic acid. The ability to scavenge 1, l-diphenyl-2-picryl-hydrazyl (DPPH) radical is measured by a decrease in the absorbance at 517 nm as described by Hou comparing with standard Ascorbic acid. Phenolic contents ranging from 35 to 175 and 30 to 85mg of Gallic acid equivalents (GAE) per gram of extract were present in S.oblonga & G.sylvestre respectively. At 100ug concentration in S.oblonga & G.sylvestre produced 81.7 & 66.8% of DPPH scavenging activity respectively and the standard ascorbic acid (10μg) produced 91.5% scavenging activity. Based on the study results, both the plant extracts of S.oblonga and G.sylvestre have significant amounts of phenolic compounds and exhibit free radical scavenging potential. However, between the two, S.oblonga plant had better antioxidant properties. Hence these herbal constituents are of adjuvant importance in the management of various free-radical mediated pathological conditions.
AB - The over production of free radicals is involved in triggering many diseases such as diabetes, cancer, rheumatoid arthritis, cirrhosis and arteriosclerosis. Antioxidants derived from plant sources offer resistance against the oxidative stress by way of their free radicals scavenging & lipid peroxidation inhibitory actions. Present study was designed to compare the free radical scavenging activity in relation to the total phenolic content of G. sylvestre & S. oblonga. Total phenolic content of the extracts was determined by using Folin-Ciocalteu method following a slightly modified method of Singleton by comparing with standard Gallic acid. The ability to scavenge 1, l-diphenyl-2-picryl-hydrazyl (DPPH) radical is measured by a decrease in the absorbance at 517 nm as described by Hou comparing with standard Ascorbic acid. Phenolic contents ranging from 35 to 175 and 30 to 85mg of Gallic acid equivalents (GAE) per gram of extract were present in S.oblonga & G.sylvestre respectively. At 100ug concentration in S.oblonga & G.sylvestre produced 81.7 & 66.8% of DPPH scavenging activity respectively and the standard ascorbic acid (10μg) produced 91.5% scavenging activity. Based on the study results, both the plant extracts of S.oblonga and G.sylvestre have significant amounts of phenolic compounds and exhibit free radical scavenging potential. However, between the two, S.oblonga plant had better antioxidant properties. Hence these herbal constituents are of adjuvant importance in the management of various free-radical mediated pathological conditions.
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M3 - Article
AN - SCOPUS:84940503103
SN - 0976-044X
VL - 33
SP - 270
EP - 273
JO - International Journal of Pharmaceutical Sciences Review and Research
JF - International Journal of Pharmaceutical Sciences Review and Research
IS - 2
M1 - 54
ER -
