TY - JOUR
T1 - Toxicological evaluation of Terminalia paniculata bark extract and its protective effect against CCl4-induced liver injury in rodents
AU - Talwar, Sahil
AU - Jagani, Hitesh V.
AU - Nayak, Pawan G.
AU - Kumar, Nitesh
AU - Kishore, Anoop
AU - Bansal, Punit
AU - Shenoy, Rekha R.
AU - Nandakumar, Krishnadas
N1 - Funding Information:
The authors would like to acknowledge Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka, India for providing the funds and facilities to carry out the work and also the financial support offered by FIST (Fund for Improvement of S&T Infrastructure in Universities and Higher Educational Institutions) sponsored programme of Department of Science & Technology, Government of India, New Delhi and Department of Biotechnology, Government of India, New Delhi for purchase of auto analyzer (Cobas C111, Fritz Hoffmann-La Roche Ltd., Basel, Switzerland) used in this study. Also, the authors would like to thank Prof. Joseph W. Lynch, Queensland Brain Institute, The University of Queensland, Australia for copyediting this manuscript.
PY - 2013/6/6
Y1 - 2013/6/6
N2 - Background: Based on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage.Methods: Intrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and sub-chronic toxicity studies in mice. TPW was then evaluated against CCl4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCl4-treated Chang cells were also elucidated.Results: TPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCl4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCl4-treated Chang cells, improving the viability considerably.Conclusion: The findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.
AB - Background: Based on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage.Methods: Intrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and sub-chronic toxicity studies in mice. TPW was then evaluated against CCl4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCl4-treated Chang cells were also elucidated.Results: TPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCl4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCl4-treated Chang cells, improving the viability considerably.Conclusion: The findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.
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U2 - 10.1186/1472-6882-13-127
DO - 10.1186/1472-6882-13-127
M3 - Article
C2 - 23742226
AN - SCOPUS:84878488721
SN - 1472-6882
VL - 13
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
M1 - 127
ER -