TY - JOUR
T1 - Types of spectroscopy and microscopy techniques for cancer diagnosis
T2 - a review
AU - Kaniyala Melanthota, Sindhoora
AU - Kistenev, Yury V.
AU - Borisova, Ekaterina
AU - Ivanov, Deyan
AU - Zakharova, Olga
AU - Boyko, Andrey
AU - Vrazhnov, Denis
AU - Gopal, Dharshini
AU - Chakrabarti, Shweta
AU - K, Shama Prasada
AU - Mazumder, Nirmal
N1 - Funding Information:
NM thank the Department of Science and Technology (DST), Government of India, for the financial support (Project Number-DST/INT/BLG/P-03/2019). NM thank Manipal Academy of Higher Education (MAHE), Manipal and Technology Information Forecasting and Assessment Council-Centre of Relevance and Excellence (TIFAC-CORE) in Pharmacogenomics, Manipal School of Life Sciences, MAHE for providing the infrastructure and facilities. SKM thank MAHE, Manipal, Karnataka, India for the Dr. T.M.A. Pai Ph.D. fellowship. EB, DI thank the financial support of Russian Science Foundation (Project No. 18-15-00139) and Bulgarian Science Fund (Project No. KP06-India-8/2019) for the results related to cancer tissues fluorescence studies. YVK thanks the financial support from the grant of the Government of the Russian Federation for state support of scientific research conducted under the supervision of leading scientists in Russian institutions of higher education, scientific foundations, and state research centres of the Russian Federation (registration number 2020-220-08-2389). YUK also thank the support of a grant in accordance with Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021). The authors thank Petr J. Mitchell, Tomsk State University, for style review.
Funding Information:
NM thank the Department of Science and Technology (DST), Government of India, for the financial support (Project Number-DST/INT/BLG/P-03/2019). NM thank Manipal Academy of Higher Education (MAHE), Manipal and Technology Information Forecasting and Assessment Council-Centre of Relevance and Excellence (TIFAC-CORE) in Pharmacogenomics, Manipal School of Life Sciences, MAHE for providing the infrastructure and facilities. SKM thank MAHE, Manipal, Karnataka, India for the Dr. T.M.A. Pai Ph.D. fellowship. EB, DI thank the financial support of Russian Science Foundation (Project No. 18-15-00139) and Bulgarian Science Fund (Project No. KP06-India-8/2019) for the results related to cancer tissues fluorescence studies. YVK thanks the financial support from the grant of the Government of the Russian Federation for state support of scientific research conducted under the supervision of leading scientists in Russian institutions of higher education, scientific foundations, and state research centres of the Russian Federation (registration number 2020-220-08-2389). YUK also thank the support of a grant in accordance with Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021). The authors thank Petr J. Mitchell, Tomsk State University, for style review.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/10
Y1 - 2022/10
N2 - Cancer is a life-threatening disease that has claimed the lives of many people worldwide. With the current diagnostic methods, it is hard to determine cancer at an early stage, due to its versatile nature and lack of genomic biomarkers. The rapid development of biophotonics has emerged as a potential tool in cancer detection and diagnosis. Using the fluorescence, scattering, and absorption characteristics of cells and tissues, it is possible to detect cancer at an early stage. The diagnostic techniques addressed in this review are highly sensitive to the chemical and morphological changes in the cell and tissue during disease progression. These changes alter the fluorescence signal of the cell/tissue and are detected using spectroscopy and microscopy techniques including confocal and two-photon fluorescence (TPF). Further, second harmonic generation (SHG) microscopy reveals the morphological changes that occurred in non-centrosymmetric structures in the tissue, such as collagen. Again, Raman spectroscopy is a non-destructive method that provides a fingerprinting technique to differentiate benign and malignant tissue based on Raman signal. Photoacoustic microscopy and spectroscopy of tissue allow molecule-specific detection with high spatial resolution and penetration depth. In addition, terahertz spectroscopic studies reveal the variation of tissue water content during disease progression. In this review, we address the applications of spectroscopic and microscopic techniques for cancer detection based on the optical properties of the tissue. The discussed state-of-the-art techniques successfully determines malignancy to its rapid diagnosis.
AB - Cancer is a life-threatening disease that has claimed the lives of many people worldwide. With the current diagnostic methods, it is hard to determine cancer at an early stage, due to its versatile nature and lack of genomic biomarkers. The rapid development of biophotonics has emerged as a potential tool in cancer detection and diagnosis. Using the fluorescence, scattering, and absorption characteristics of cells and tissues, it is possible to detect cancer at an early stage. The diagnostic techniques addressed in this review are highly sensitive to the chemical and morphological changes in the cell and tissue during disease progression. These changes alter the fluorescence signal of the cell/tissue and are detected using spectroscopy and microscopy techniques including confocal and two-photon fluorescence (TPF). Further, second harmonic generation (SHG) microscopy reveals the morphological changes that occurred in non-centrosymmetric structures in the tissue, such as collagen. Again, Raman spectroscopy is a non-destructive method that provides a fingerprinting technique to differentiate benign and malignant tissue based on Raman signal. Photoacoustic microscopy and spectroscopy of tissue allow molecule-specific detection with high spatial resolution and penetration depth. In addition, terahertz spectroscopic studies reveal the variation of tissue water content during disease progression. In this review, we address the applications of spectroscopic and microscopic techniques for cancer detection based on the optical properties of the tissue. The discussed state-of-the-art techniques successfully determines malignancy to its rapid diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=85134338102&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85134338102&partnerID=8YFLogxK
U2 - 10.1007/s10103-022-03610-3
DO - 10.1007/s10103-022-03610-3
M3 - Review article
C2 - 35834141
AN - SCOPUS:85134338102
SN - 0268-8921
VL - 37
SP - 3067
EP - 3084
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 8
ER -
