TY - JOUR
T1 - Understanding the effects of Abhraka Bhasma on genotoxicity and its DNA repair potential in mouse model
AU - Kulala, Divya S.
AU - Prasad, Keshava
AU - Reddy, Poojitha S.
AU - Maruthiyodan, Swathi
AU - Joshi, Manjunath B.
AU - Satyamoorthy, Kapaettu
AU - Guruprasad, Kanive P.
N1 - Funding Information:
The study was funded by Vision Group of Science and Technology (VGST), Government of Karnataka [No: CESEM-GRD 179).
Funding Information:
Authors thank TIFAC-CORE, Government of India and Manipal Academy for Higher Education, Manipal for infrastructure and Centre for Ayurvedic Biology for valuable inputs. We gratefully acknowledge the financial support from Vision Group of Science and Technology ( VGST ), Government of Karnataka (Grant No: CESEM-GRD 179). KP received the Joint CSIR-UGC fellowship under UGC scheme.
Funding Information:
Authors thank TIFAC-CORE, Government of India and Manipal Academy for Higher Education, Manipal for infrastructure and Centre for Ayurvedic Biology for valuable inputs. We gratefully acknowledge the financial support from Vision Group of Science and Technology (VGST), Government of Karnataka (Grant No: CESEM-GRD 179). KP received the Joint CSIR-UGC fellowship under UGC scheme.
Publisher Copyright:
© 2022 The Authors
PY - 2022
Y1 - 2022
N2 - Background: Metal toxicity is of major concern to human health. The metals may modulate molecular mechanisms of various pathways. Rasashastra, the branch of Ayurveda, narrates the properties, unique preparation, processing techniques, and therapeutic uses of minerals. The use of herbal metallic preparations has evoked concern for their potential to produce toxicity, interest in efficacy as therapeutic agents and safety related issues. Abhraka Bhasma, is one such incinerated herbo-metallic preparation of mica, widely used by traditional medicine practitioners. Although there are reports of Abhraka Bhasma on beneficial effects, clear evidence is lacking on the effect of Abhraka Bhasma on genotoxicity and DNA repair. Objective: The present study aims to understand the effects of Abhraka Bhasma on geno toxicity, DNA repair, and other mechanisms in the mice test model. Material and methods: The experiments were conducted in in vivo Swiss albino mice. The acute oral toxicity was performed as per the OECD guidelines. The mice were treated with Abhraka Bhasma (120 or 360 mg/kg body weight) for 7 days. They were then challenged with ethyl methanesulfonate and the DNA repair was analyzed. Results: The data obtained indicated that the Abhraka Bhasma is not a genotoxic and reproductive toxic formulation. The selected higher concentration of Abhraka Bhasma showed a protective role against ethyl methanesulfonate induced chromosomal damages and enhanced constitutive DNA base excision repair in mice. Conclusion: The anti-oxidant, potentiation of DNA repair and hematinic properties of Abhraka Bhasma may be attributed to the synergistic actions of its bioactive components.
AB - Background: Metal toxicity is of major concern to human health. The metals may modulate molecular mechanisms of various pathways. Rasashastra, the branch of Ayurveda, narrates the properties, unique preparation, processing techniques, and therapeutic uses of minerals. The use of herbal metallic preparations has evoked concern for their potential to produce toxicity, interest in efficacy as therapeutic agents and safety related issues. Abhraka Bhasma, is one such incinerated herbo-metallic preparation of mica, widely used by traditional medicine practitioners. Although there are reports of Abhraka Bhasma on beneficial effects, clear evidence is lacking on the effect of Abhraka Bhasma on genotoxicity and DNA repair. Objective: The present study aims to understand the effects of Abhraka Bhasma on geno toxicity, DNA repair, and other mechanisms in the mice test model. Material and methods: The experiments were conducted in in vivo Swiss albino mice. The acute oral toxicity was performed as per the OECD guidelines. The mice were treated with Abhraka Bhasma (120 or 360 mg/kg body weight) for 7 days. They were then challenged with ethyl methanesulfonate and the DNA repair was analyzed. Results: The data obtained indicated that the Abhraka Bhasma is not a genotoxic and reproductive toxic formulation. The selected higher concentration of Abhraka Bhasma showed a protective role against ethyl methanesulfonate induced chromosomal damages and enhanced constitutive DNA base excision repair in mice. Conclusion: The anti-oxidant, potentiation of DNA repair and hematinic properties of Abhraka Bhasma may be attributed to the synergistic actions of its bioactive components.
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U2 - 10.1016/j.jaim.2022.100598
DO - 10.1016/j.jaim.2022.100598
M3 - Article
AN - SCOPUS:85136763926
SN - 0975-9476
VL - 14
JO - Journal of Ayurveda and Integrative Medicine
JF - Journal of Ayurveda and Integrative Medicine
IS - 2
M1 - 100598
ER -