TY - JOUR
T1 - Validation of a sensitive and robust reversed phase-HPLC method for determination of temozolomide
AU - Attari, Zenab
AU - Kumar, Lalit
AU - Rao, C. Mallikarjuna
AU - Koteshwara, K. B.
PY - 2016
Y1 - 2016
N2 - Temozolomide is a physiologically pH labile, alkylating agent used in glioblastoma multiforme. An attempt has been made to develop and validate a simple, sensitive, accurate, precise and robust HPLC method for quantification of temozolomide. Ammonium formate and acetonitrile combination used as mobile phase using BDS C18 column resulted in elution of temozolomide with retention time of 5.866 min. The various parameters, precision, accuracy and robustness of the method were evaluated. The % RSD of the parameters were acceptable (< 2 or/and 1%). The LOD and LOQ were found to be 13.13 and 39.81 ng/mL, respectively. The recovery of the drug was observed to be 99.97 to 100.93%.
AB - Temozolomide is a physiologically pH labile, alkylating agent used in glioblastoma multiforme. An attempt has been made to develop and validate a simple, sensitive, accurate, precise and robust HPLC method for quantification of temozolomide. Ammonium formate and acetonitrile combination used as mobile phase using BDS C18 column resulted in elution of temozolomide with retention time of 5.866 min. The various parameters, precision, accuracy and robustness of the method were evaluated. The % RSD of the parameters were acceptable (< 2 or/and 1%). The LOD and LOQ were found to be 13.13 and 39.81 ng/mL, respectively. The recovery of the drug was observed to be 99.97 to 100.93%.
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M3 - Article
AN - SCOPUS:84964905137
SN - 0326-2383
VL - 35
SP - 967
EP - 971
JO - Latin American Journal of Pharmacy
JF - Latin American Journal of Pharmacy
IS - 5
ER -