TY - JOUR
T1 - Wavelength modulation based surface plasmon resonance sensor for detection of cardiac marker proteins troponin I and troponin T
AU - Sinha, Rajeev K.
N1 - Funding Information:
Financial support from Department of Science and Technology (DST) India under the project Grant No. IDP/BDTD/11/2019 is gratefully acknowledged.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/12/1
Y1 - 2021/12/1
N2 - A wavelength modulation based surface plasmon resonance (SPR) sensor setup was developed with inexpensive components. The developed setup is utilized for the detection of cardiac marker proteins, cardiac troponin I (CTnI), and cardiac troponin T (CTnT). A simple approach was used for the fabrication of CTnI/CTnT antibody sensor chip. A self-assembled monolayer (SAM) of 1-octanethiol and 11-mercaptoundecanoic acid (11-MUA) was formed on annealed Au thin film. The −COOH group of 11-MUA was modified with EDC-NHS for amine coupling to monoclonal antibodies of CTnI and CTnT. The developed SPR setup and the sensor chip with monoclonal antibodies showed good performance for the detection of CTnI and CTnT in direct assay. For CTnI, the lowest concentration detected was 0.03125 ng/mL whereas for CTnT, it was 0.0625 μg/mL. The detected concentrations are well below the onset concentration of these two proteins in blood following the acute myocardial infarction (AMI). The performance of this direct assay based on the developed setup and sensor chip therefore can be used easily for detection of CTnI and CTnT during their onset period.
AB - A wavelength modulation based surface plasmon resonance (SPR) sensor setup was developed with inexpensive components. The developed setup is utilized for the detection of cardiac marker proteins, cardiac troponin I (CTnI), and cardiac troponin T (CTnT). A simple approach was used for the fabrication of CTnI/CTnT antibody sensor chip. A self-assembled monolayer (SAM) of 1-octanethiol and 11-mercaptoundecanoic acid (11-MUA) was formed on annealed Au thin film. The −COOH group of 11-MUA was modified with EDC-NHS for amine coupling to monoclonal antibodies of CTnI and CTnT. The developed SPR setup and the sensor chip with monoclonal antibodies showed good performance for the detection of CTnI and CTnT in direct assay. For CTnI, the lowest concentration detected was 0.03125 ng/mL whereas for CTnT, it was 0.0625 μg/mL. The detected concentrations are well below the onset concentration of these two proteins in blood following the acute myocardial infarction (AMI). The performance of this direct assay based on the developed setup and sensor chip therefore can be used easily for detection of CTnI and CTnT during their onset period.
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U2 - 10.1016/j.sna.2021.113104
DO - 10.1016/j.sna.2021.113104
M3 - Article
AN - SCOPUS:85115109708
SN - 0924-4247
VL - 332
JO - Sensors and Actuators A: Physical
JF - Sensors and Actuators A: Physical
M1 - 113104
ER -