Withaferin A: A new radiosensitizer from the Indian medicinal plant withania somnifera

P. Uma Devi; K. Akagi; V. Ostapenko; Y. Tanaka; T. Sugahara

doi:10.1080/095530096146020

Withaferin A: A new radiosensitizer from the Indian medicinal plant withania somnifera

P. Uma Devi^*
, K. Akagi
, V. Ostapenko
, Y. Tanaka
, T. Sugahara

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD₅₀ for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31% survival and 1.4 for 10% survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G₂-M block, with a maximum accumulation of cells in G₂-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.

Original language	English
Pages (from-to)	193-197
Number of pages	5
Journal	International Journal of Radiation Biology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1080/095530096146020
Publication status	Published - 01-05-1996
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Radiological and Ultrasound Technology
Radiology Nuclear Medicine and imaging

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10.1080/095530096146020

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title = "Withaferin A: A new radiosensitizer from the Indian medicinal plant withania somnifera",

abstract = "Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD50 for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31\% survival and 1.4 for 10\% survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G2-M block, with a maximum accumulation of cells in G2-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.",

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T2 - A new radiosensitizer from the Indian medicinal plant withania somnifera

AU - Uma Devi, P.

AU - Akagi, K.

AU - Ostapenko, V.

AU - Tanaka, Y.

AU - Sugahara, T.

PY - 1996/5/1

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N2 - Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD50 for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31% survival and 1.4 for 10% survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G2-M block, with a maximum accumulation of cells in G2-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.

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Withaferin A: A new radiosensitizer from the Indian medicinal plant withania somnifera

Abstract

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